RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-ATF7 Antibody

Catalog #: 144-07580

ATF7 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P17544
Gene Id
11016
Gene Symbols
ATF7
Protein Name / Synonyms
ATF7, ATFA, activating transcription factor 7
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
13kDa/33kDa/49kDa/50kDa/51kDa/52kDa
Observed MW
53kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human ATF7 (NP_001193611.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATF7 antibody (144-07580) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ATF7 antibody (144-07580)

Immunofluorescence analysis of A549 cells using ATF7 antibody (144-07580). Blue: DAPI for nuclear staining.
Immunofluorescence - ATF7 antibody (144-07580)

Expiration:
12 months from the date of shipment when stored properly.