Anti-ATG13 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O75143
Gene Id	9776
Gene Symbols	ATG13
Protein Name / Synonyms	ATG13, KIAA0652, PARATARG8, autophagy related 13
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is an autophagy factor and a target of the TOR kinase signaling pathway. The encoded protein is essential for autophagosome formation and mitophagy.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

44kDa/52kDa/56kDa/60kDa

Observed MW

70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 281-480 of human ATG13 (NP_055556.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATG13 antibody (144-00690) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Western blot - ATG13 Polyclonal Antibody (144-00690)

Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody (144-00690) at dilution of 1:100 (40x lens).
Immunohistochemistry - ATG13 Polyclonal Antibody (144-00690)

Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody (144-00690) at dilution of 1:100 (40x lens).
Immunohistochemistry - ATG13 Polyclonal Antibody (144-00690)

Immunofluorescence analysis of A549 cells using ATG13 antibody (144-00690). Blue: DAPI for nuclear staining.
Immunofluorescence - ATG13 Polyclonal Antibody (144-00690)

Expiration:

12 months from the date of shipment when stored properly.

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