Anti-ATG16L Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q676U5
Gene Id	55054
Gene Symbols	ATG16L1
Protein Name / Synonyms	ATG16L
Clonality	Polyclonal
Preparation	This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-190 amino acids from human ATG16L.
Concentration (lot specific)	0.310 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.

Specificity

Other Names

ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1

NCBI Accession #

NP_001177195.1;NP_001177196.1;NP_060444.3;NP_110430.5;NP_942593.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

68265

Recommended Dilutions

IF: 1:25; WB: 1:1000; IHC: 1:10-50

	Immunofluorescent analysis of U251 cells, using ATG16L Antibody(Cat# 102-13715). U251 cells(right) were treated with Chloroquine (50 µM,16h). 102-13715 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
	APG16L Antibody (Cat# 102-13715) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the APG16L antibody detected the APG16L protein (arrow).
	Western blot of APG16L (L92) Pab (Cat# 102-13715) in mouse brain tissue lysate.
	Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Cos7, HEK293, MEF, and Hela cells, left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.