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Anti-ATG3 (C-term) Antibody
Catalog #: 102-13655
Rabbit Anti-Human ATG3 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9NT62 |
| Gene Id | 64422 |
| Gene Symbols | ATG3 |
| Protein Name / Synonyms | ATG3 (C-term) |
| Clonality | Polyclonal |
| Preparation | This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-112 amino acids from the C-terminal region of human ATG3. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine
Other Names
ATG3; APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; Protein PC3-96
NCBI Accession #
NP_001265641.1;NP_071933.2
Other Accession #
Q6AZ50; Q9CPX6; Q0VCL3
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35864
Recommended Dilutions
WB: 1:1000
 | APG3L Antibody (I98) (Cat# 102-13655) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APG3L antibody detected the APG3L protein (arrow). |
 | Western blot analysis of APG3L (arrow) using rabbit polyclonal APG3L Antibody (I98) (Cat# 102-13655). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG3L gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.