Anti-ATG3 (C-term K183) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9NT62
Gene Id	64422
Gene Symbols	ATG3
Protein Name / Synonyms	ATG3 (C-term K183)
Clonality	Polyclonal
Preparation	This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the C-terminal region of human ATG3.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Specificity

Predicted Reactivity

Bovine

Other Names

ATG3; APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; Protein PC3-96

NCBI Accession #

NP_001265641.1;NP_071933.2

Other Accession #

Q0VCL3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

35864

Recommended Dilutions

WB: 1:1000

Human APG3L was detected using purified polyclonal antibody 102-13656 in Western blot on mouse colon tissue lysate.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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