Anti-ATG4B Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9Y4P1
Gene Id	23192
Gene Symbols	ATG4B
Protein Name / Synonyms	ATG4B
Clonality	Polyclonal
Preparation	This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-284 amino acids from human ATG4B.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3 to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.

Specificity

Predicted Reactivity

Mouse, Zebrafish, Bovine, Chicken

Other Names

ATG4B; APG4B; AUTL1; KIAA0943; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B

NCBI Accession #

NP_037457.3;NP_847896.1

Other Accession #

Q8BGE6; Q6DG88; Q6PZ02; Q6PZ03

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

44294

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of APG4B (arrow) using purified Pab (Cat# 102-13670). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Autophagy APG4B Antibody (E273)(Cat# 102-13670), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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