Anti-ATG5 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9H1Y0
Gene Id	9474
Gene Symbols	ATG5
Protein Name / Synonyms	ATG5 (N-term)
Clonality	Polyclonal
Preparation	This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG5.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Specificity

Predicted Reactivity

Rat, Bovine, Pig

Other Names

ATG5; APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein

NCBI Accession #

NP_004840.1

Other Accession #

Q3MQ06; Q3MQ04; Q99J83; Q3MQ24

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

32447

Recommended Dilutions

WB: 1:1000; IF: 1:200

	Western blot analysis of APG5L Antibody (D3) (arrow) using rabbit polyclonal APG5L Antibody (D3) (Cat# 102-13690). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.
	Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-13690 ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
	APG5L Antibody (D3) (Cat# 102-13690) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG5L antibody detected the APG5L protein (arrow).
	The anti-APG5L Pab (Cat# 102-13690) is used in Western blot to detect APG5L in mouse brain tissue lysate.
	Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (D3) (Cat# 102-13690). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.