Anti-ATM Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13315
Gene Id	472
Gene Symbols	ATM
Protein Name / Synonyms	ATM, AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1, serine-protein kinase ATM
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease, Cancer, Autophagy, DNA Damage
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.

Dilutions

WB 1:500 - 1:1000

Format

Calculated MW

350kDa

Observed MW

310kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1900-2000 of human ATM (NP_000042.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of H460 cells, using ATM Antibody (144-05908) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ATM Antibody (144-05908)

Expiration:

12 months from the date of shipment when stored properly.

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