Anti-ATOH1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q92858
Gene Id	474
Gene Symbols	ATOH1
Protein Name / Synonyms	ATOH1, ATH1, HATH1, MATH-1, bHLHa14, protein atonal homolog 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

38kDa

Observed MW

35kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human ATOH1 (NP_005163.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATOH1 Antibody (144-06530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - ATOH1 Polyclonal Antibody (144-06530)

Immunofluorescence analysis of MCF7 cells using ATOH1 antibody (144-06530). Blue: DAPI for nuclear staining.
Immunofluorescence - ATOH1 Polyclonal Antibody (144-06530)

Expiration:

12 months from the date of shipment when stored properly.

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