Anti-ATP2A1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O14983
Gene Id	487
Gene Symbols	ATP2A1
Protein Name / Synonyms	ATP2A1 (C-term)
Clonality	Polyclonal
Preparation	This ATP2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 974-1001 amino acids from the C-terminal region of human ATP2A1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ATP2A1 encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction.

Specificity

Predicted Reactivity

Rabbit

Other Names

ATP2A1; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase

NCBI Accession #

NP_004311.1;NP_775293.1

Other Accession #

P04191

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

110252

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of lysates from RD,HT1080 cell line (from left to right),using ATP2A1 Antibody (C-term)(Cat# 102-15791).102-15791 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
	ATP2A1 Antibody (C-term) (Cat# 102-15791) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP2A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	ATP2A1 Antibody (C-term) (Cat# 102-15791) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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