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ISO 13485:2016 cGMP

Anti-ATP5C1 Antibody

Catalog #: 144-60964

ATP5C1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P36542
Gene Id
509
Gene Symbols
ATP5C1
Protein Name / Synonyms
ATP5C1, ATP5C, ATP5CL1, ATP synthase subunit gamma, mitochondrial
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW
32kDa
Observed MW
36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-298 of human ATP5C1 (NP_001001973.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATP5C1 antibody (144-60964) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5S.
Western blot - ATP5C1 antibody (144-60964)

Expiration:
12 months from the date of shipment when stored properly.