Anti-ATP5D (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P30049\|P35434\|Q9D3D9\|P05630
Gene Id	513
Gene Symbols	ATP5F1D
Protein Name / Synonyms	ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit, ATP5D
Clonality	Polyclonal
Clone	RB48628
Isotype	Kappa, Rabbit
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Other Information

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Calculated Molecular Weight (Da)

17490

Antigen Source

Human

Images

WB (1:1000-1:4000)

Western blot analysis of lysates from A549, HepG2, Jurkat cell line, mouse brain tissue lysate, rat PC-12 cell line (from left to right), using ATP5D Antibody (C-term)(Cat. #102-24083). 102-24083 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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