Anti-ATP5H (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O75947
Gene Id	10476
Gene Symbols	ATP5H
Protein Name / Synonyms	ATP5H (Center)
Clonality	Polyclonal
Preparation	This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human ATP5H.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IHC-P, FC, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

Specificity

Predicted Reactivity

Bovine

Other Names

ATP5H; ATP synthase subunit d, mitochondrial

NCBI Accession #

NP_001003785.1;NP_006347.1

Other Accession #

P13620; NP_001003785.1; NP_006347.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

18491

Recommended Dilutions

IHC: 1:50-100; FC: 1:10-50; WB: 1:1000

	ATP5H antibody (Center) (Cat# 102-10584) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	ATP5H Antibody (Center) (Cat# 102-10584) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	ATP5H Antibody (Center) (Cat# 102-10584) western blot analysis in HepG2,NCI-H460,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ATP5H antibody detected the ATP5H protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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