RayBiotech

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ISO 13485:2016 cGMP

Anti-ATP5J Antibody

Catalog #: 144-65734

ATP5J pAb

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P18859
Gene Id
522
Gene Symbols
ATP5J
Protein Name / Synonyms
ATP5J, ATP5, ATP5A, ATPM, CF6, F6, ATP synthase-coupling factor 6, mitochondrial
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.

Dilutions

WB 1:200 - 1:2000; IF 1:50 - 1:200

Format

Calculated MW
12kDa/13kDa
Observed MW
13kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human ATP5J (NP_001676.2).

Modification

Unmodified

Storage

Store at -20°. Avoid freeze / thaw cycles.

Images

Western blot

Expiration:
12 months from the date of shipment when stored properly.