Anti-ATP5L Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O75964
Gene Id	10632
Gene Symbols	ATP5L
Protein Name / Synonyms	ATP5L, ATP5JG, ATP synthase subunit g, mitochondrial
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

11kDa

Observed MW

12kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATP5L antibody (144-09178) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - ATP5L antibody (144-09178)

Expiration:

12 months from the date of shipment when stored properly.

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