Anti-ATP5O Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P48047
Gene Id	539
Gene Symbols	ATP5O
Protein Name / Synonyms	ATP5O, ATPO, HMC08D05, OSCP, ATP synthase subunit O, mitochondrial
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. The encoded protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.

Dilutions

WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

23kDa

Observed MW

23kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-213 of human ATP5O (NP_001688.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATP5O antibody (144-08103) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ATP5O Polyclonal Antibody (144-08103)

Immunofluorescence analysis of U2OS cells using ATP5O antibody (144-08103) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - ATP5O Polyclonal Antibody (144-08103)

Expiration:

12 months from the date of shipment when stored properly.