Anti-ATP6V0B (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q99437
Gene Id	533
Gene Symbols	ATP6V0B
Protein Name / Synonyms	ATP6V0B (Center)
Clonality	Polyclonal
Preparation	This ATP6V0B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human ATP6V0B.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.

Specificity

Predicted Reactivity

Bovine

Other Names

ATP6V0B; ATP6F; V-type proton ATPase 21 kDa proteolipid subunit; Vacuolar proton pump 21 kDa proteolipid subunit; hATPL

NCBI Accession #

NP_001034546.1;NP_004038.1

Other Accession #

Q2TA24; NP_001034546.1; NP_004038.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

21406

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	ATP6V0B Antibody (Center) (Cat# 102-10647) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EKI2 antibody detected the EKI2 protein (arrow).
	ATP6V0B Antibody (Center) (Cat# 102-10647) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP6V0B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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