Anti-ATP6V1E1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P36543
Gene Id	529
Gene Symbols	ATP6V1E1
Protein Name / Synonyms	ATP6V1E1, ATP6E, ATP6E2, ATP6V1E, P31, Vma4, ARCL2C, V-type proton ATPase subunit E 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.

Dilutions

WB 1:1000 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

22kDa/23kDa/26kDa

Observed MW

35kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (144-03756) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot - ATP6V1E1 Polyclonal Antibody (144-03756)

Expiration:

12 months from the date of shipment when stored properly.

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