Anti-ATP7B Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P35670
Gene Id	540
Gene Symbols	ATP7B
Protein Name / Synonyms	ATP7B, PWD, WC1, WD, WND, copper-transporting ATPase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

133kDa/145kDa/155kDa/157kDa

Observed MW

150kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1055-1354 of human ATP7B (NP_001230111.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATP7B antibody (144-05676) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - ATP7B antibody (144-05676)

Immunofluorescence analysis of U2OS cells using ATP7B antibody (144-05676). Blue: DAPI for nuclear staining.
Immunofluorescence - ATP7B antibody (144-05676)

Expiration:

12 months from the date of shipment when stored properly.

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