Anti-ATRIP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q8WXE1
Gene Id	84126
Gene Symbols	ATRIP
Protein Name / Synonyms	ATRIP, ATR-interacting protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:200

Format

Calculated MW

72kDa/76kDa/83kDa/85kDa

Observed MW

86kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATRIP antibody (144-07139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Western blot - ATRIP antibody (144-07139)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (144-07139). Western blot was performed from the immunoprecipitate using ATRIP antibody (144-07139) at a dilition of 1:1000.
Immunoprecipitation - 1ug ATRIP antibody (144-07139)

Expiration:

12 months from the date of shipment when stored properly.

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