RayBiotech

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ISO 13485:2016 cGMP

Anti-AURKA Antibody

Catalog #: 144-02121

AURKA Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
O14965
Gene Id
6790
Gene Symbols
AURKA
Protein Name / Synonyms
AURKA, AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7, aurora kinase A
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
45kDa
Observed MW
46kDa

Immunogen

A synthetic peptide of human AURKA

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AURKA antibody (144-02121) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - AURKA antibody (144-02121)

Expiration:
12 months from the date of shipment when stored properly.