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Anti-BAD Antibody
Catalog #: 130-10024
Rabbit Anti-Human BAD Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q92934 |
| Gene Id | 572 |
| Gene Symbols | BAD BBC6 BCL2L8 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human BAD. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000(at least detecting 25ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Human BAD protein is a member of the Bcl-2 family of apoptosis related proteins. BAD acts as a cell death promoter, and may form heterodimers with Bcl-2 or with Bcl-XL. BAD promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 By similarity. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat BAD has not yet been tested.
Storage
Keep it at 4°C if intended for use within a month, otherwise, Store at -20°C. Minimize freezing and thawing when use.
Related Products
Recombinant Human BAD (Cat# 228-10089-3 )Goat anti ABAD / HADH2, with HRP-conjugated secondary antibody (Cat#ER-14-0034) Rabbit Anti-Human BAD (Cat#DS-PB-00222)
References
- Popivanova BK, et.al.,(2007) Proto-oncogene, Pim-3 with serine/threonine kinase activity, is aberrantly expressed in human colon cancer cells and can prevent Bad-mediated apoptosis. Cancer Sci. 98(3):321-8.
- Petros AM, et.al., (2000) Rationale for Bcl-xL/Bad peptide complex formation from structure, mutagenesis, and biophysical studies. Protein Sci. 9(12):2528-34.
Expiration:
12 months from the date of shipment when stored properly.