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Anti-BAD Antibody, Biotinylated
Catalog #: 130-10024B-50
Rabbit anti-BAD Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q92934 |
| Gene Id | 572 |
| Gene Symbols | BAD BBC6 BCL2L8 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human BAD. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000(at least detecting 25 µg/ml)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Human BAD protein is a member of the Bcl-2 family of apoptosis related proteins. BAD acts as a cell death promoter, and may form heterodimers with Bcl-2 or with Bcl-XL. BAD promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 By similarity. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat BAD has not yet been tested.
Related Products
Recombinant Human BAD (Cat# 228-10089-3 )Goat anti ABAD / HADH2, with HRP-conjugated secondary antibody (Cat#ER-14-0034) Rabbit Anti-Human BAD (Cat#DS-PB-00222)
References
- Popivanova BK, et.al.,(2007) Proto-oncogene, Pim-3 with serine/threonine kinase activity, is aberrantly expressed in human colon cancer cells and can prevent Bad-mediated apoptosis. Cancer Sci. 98(3):321-8.
- Petros AM, et.al., (2000) Rationale for Bcl-xL/Bad peptide complex formation from structure, mutagenesis, and biophysical studies. Protein Sci. 9(12):2528-34.
Expiration:
12 months from the date of shipment when stored properly.