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Anti-BAG1 Antibody
Catalog #: 144-01104
BAG1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q99933 |
| Gene Id | 573 |
| Gene Symbols | BAG1 |
| Protein Name / Synonyms | BAG1, BAG-1, HAP, RAP46, BCL2 associated athanogene 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IHC 1:50 - 1:200
IF 1:20 - 1:100
Format
Calculated MW
25kDa/31kDa/34kDa/38kDa
Observed MW
43kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BAG1 (NP_001165886.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BAG1 antibody (144-01104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney cancer using BAG1 Antibody (144-01104) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney injury using BAG1 Antibody (144-01104) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using BAG1 Antibody (144-01104) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using BAG1 Antibody (144-01104) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BAG1 antibody (144-01104). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.