Anti-BAG2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O95816
Gene Id	9532
Gene Symbols	BAG2
Protein Name / Synonyms	BAG2, BAG-2, dJ417I1.2, BCL2 associated athanogene 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

20kDa/23kDa

Observed MW

24kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using BAG2 antibody (144-07763) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - BAG2 Polyclonal Antibody (144-07763)

Immunohistochemistry of paraffin-embedded human kidney using BAG2 antibody (144-07763) at dilution of 1:100 (40x lens).
Immunohistochemistry - BAG2 Polyclonal Antibody (144-07763)

Immunofluorescence analysis of U2OS cells using BAG2 antibody (144-07763) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - BAG2 Polyclonal Antibody (144-07763)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products