Anti-BAG5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UL15
Gene Id	9529
Gene Symbols	BAG5
Protein Name / Synonyms	BAG5, BAG-5, BCL2 associated athanogene 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

51kDa/56kDa

Observed MW

48kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 198-447 of human BAG5 (NP_004864.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using BAG5 antibody (144-05794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - BAG5 antibody (144-05794)

Immunohistochemistry of paraffin-embedded human stomach using BAG5 antibody (144-05794) at dilution of 1:100 (40x lens).
Immunohistochemistry - BAG5 antibody (144-05794)

Immunohistochemistry of paraffin-embedded rat brain using BAG5 antibody (144-05794) at dilution of 1:100 (40x lens).
Immunohistochemistry - BAG5 antibody (144-05794)

Expiration:

12 months from the date of shipment when stored properly.

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