Anti-BAK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16611
Gene Id	578
Gene Symbols	BAK1
Protein Name / Synonyms	BAK1, BAK, BAK-LIKE, BCL2L7, CDN1, BCL2 antagonist/killer 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Apoptosis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

16kDa/23kDa

Observed MW

26kDa

Immunogen

A synthetic peptide of human BAK1

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using BAK1 Antibody (144-00204) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - BAK1 Polyclonal Antibody (144-00204)

Immunofluorescence analysis of HeLa cells using BAK1 antibody (144-00204). Blue: DAPI for nuclear staining.
Immunofluorescence - BAK1 Polyclonal Antibody (144-00204)

Expiration:

12 months from the date of shipment when stored properly.

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