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Anti-Basic Cytokeratin Antibody, Sodium Azide Free
Catalog #: 188-10214-SAF
Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken Basic Cytokeratin Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P04264 |
| Gene Id | 3848 |
| Gene Symbols | KRT1,KRTA |
| Protein Name / Synonyms | Keratin, type II cytoskeletal 1 (67 kDa cytokeratin) (Cytokeratin-1) (CK-1) (Hair alpha protein) (Keratin-1) (K1) (Type-II keratin Kb1) |
| Immunogen | Solubilized cytokeratin extract from human stratum corneum was used as the immunogen. |
| Isotype | IgG1 |
| Purity | Protein G purified |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | FACS: 0.5-1ug/million cells IF: 1-2ug/ml Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (Cat No V2330).
Storage
Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.