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Anti-Basic Cytokeratin Antibody, Sodium Azide Free
Catalog #: 188-11243-SAF
Rabbit anti-Human Recombinant Basic Cytokeratin Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q01546 |
| Gene Id | 51350 |
| Gene Symbols | KRT76,KRT2B,KRT2P |
| Protein Name / Synonyms | Keratin, type II cytoskeletal 2 oral (Cytokeratin-2P) (CK-2P) (K2P) (Keratin-76) (K76) (Type-II keratin Kb9) |
| Immunogen | Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody. |
| Isotype | IgG, Kappa |
| Purity | Protein A affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Western blot: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) |
| Domain / Region / Terminus | Cytoplasmic |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Storage
Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.