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Anti-Bax Antibody, Sodium Azide Free
Catalog #: 188-10005-SAF
Mouse anti-human/monkey Bax Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | The antibody reacts with human and monkey samples. Does not react with mouse and rat. Other species not known. |
| Host Species | Mouse |
| Accession Number | Q07812 |
| Gene Id | 581 |
| Gene Symbols | BAX,BCL2L4 |
| Protein Name / Synonyms | Apoptosis regulator BAX (Bcl-2-like protein 4) (Bcl2-L-4) |
| Immunogen | Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody. |
| Isotype | IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | ELISA: order BSA-free format for coating FACS: 0.5-1ug/million cells IF: 1-2ug/ml Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This antibody recognizes a protein of 21kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.
Storage
Store the Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.