Anti-BAZ1B Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UIG0
Gene Id	9031
Gene Symbols	BAZ1B
Protein Name / Synonyms	BAZ1B, WBSCR10, WBSCR9, WSTF, tyrosine-protein kinase BAZ1B
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

170kDa

Observed MW

190kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BAZ1B (NP_115784.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using BAZ1B antibody (144-09851) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody (144-09851) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Immunofluorescence analysis of HeLa cells using BAZ1B antibody (144-09851) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] BAZ1B Polyclonal Antibody (144-09851)

Expiration:

12 months from the date of shipment when stored properly.

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