Anti-BCAM Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse
Host Species	Rabbit
Accession Number	P50895
Gene Id	4059
Gene Symbols	BCAM
Protein Name / Synonyms	BCAM, AU, CD239, LU, MSK19, basal cell adhesion molecule
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

67kDa

Observed MW

90kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 352-547 of human BCAM (NP_005572.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse lung, using BCAM antibody (144-60773) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - BCAM antibody (144-60773)

Expiration:

12 months from the date of shipment when stored properly.

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