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Anti-BCAP31 Antibody
Catalog #: 144-07056
BCAP31 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P51572 |
| Gene Id | 10134 |
| Gene Symbols | BCAP31 |
| Protein Name / Synonyms | BCAP31, 6C6-AG, BAP31, CDM, DDCH, DXS1357E, B-cell receptor-associated protein 31 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
27kDa/34kDa
Observed MW
28kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 127-246 of human BCAP31 (NP_001243376.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BCAP31 antibody (144-07056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot analysis of extracts of various cells, using BCAP31 antibody (144-07056) at 1:500 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using BCAP31 antibody (144-07056). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.