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Anti-BCAS3 Antibody
Catalog #: 144-07274
BCAS3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9H6U6 |
| Gene Id | 54828 |
| Gene Symbols | BCAS3 |
| Protein Name / Synonyms | BCAS3, GAOB1, MAAB, breast carcinoma-amplified sequence 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
74kDa/99kDa/101kDa/102kDa/103kDa
Observed MW
115kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 674-913 of human BCAS3 (NP_060149.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BCAS3 antibody (144-07274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney cancer using BCAS3 antibody (144-07274) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using BCAS3 antibody (144-07274) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using BCAS3 antibody (144-07274) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using BCAS3 antibody (144-07274). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug BCAS3 antibody (144-07274). Western blot was performed from the immunoprecipitate using BCAS3 antibody (144-07274) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.