RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-BCAT2 Antibody

Catalog #: 144-07426

BCAT2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
O15382
Gene Id
587
Gene Symbols
BCAT2
Protein Name / Synonyms
BCAT2, BCAM, BCATM, BCT2, PP18, branched chain amino acid transaminase 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
33kDa/44kDa
Observed MW
40kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human BCAT2 (NP_001181.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using BCAT2 Antibody (144-07426) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - BCAT2 Polyclonal Antibody (144-07426)

Expiration:
12 months from the date of shipment when stored properly.