Anti-BCHE Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P06276
Gene Id	590
Gene Symbols	BCHE
Protein Name / Synonyms	BCHE, CHE1, CHE2, E1, cholinesterase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

68kDa

Observed MW

67kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 29-270 of human BCHE (NP_000046.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using BCHE antibody (144-01460) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - BCHE Polyclonal Antibody (144-01460)

Immunohistochemistry of paraffin-embedded rat kidney using BCHE antibody (144-01460) at dilution of 1:100 (40x lens).
Immunohistochemistry - BCHE Polyclonal Antibody (144-01460)

Immunofluorescence analysis of U2OS cells using BCHE antibody (144-01460). Blue: DAPI for nuclear staining.
Immunofluorescence - BCHE Polyclonal Antibody (144-01460)

Expiration:

12 months from the date of shipment when stored properly.

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