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Anti-BCKDK Antibody
Catalog #: 144-08184
BCKDK Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | O14874 |
| Gene Id | 10295 |
| Gene Symbols | BCKDK |
| Protein Name / Synonyms | BCKDK, BCKDKD, BDK, branched chain ketoacid dehydrogenase kinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
37kDa/41kDa/46kDa
Observed MW
46kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 153-412 of human BCKDK (NP_005872.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BCKDK antibody (144-08184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using BCKDK antibody (144-08184) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using BCKDK antibody (144-08184) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using BCKDK antibody (144-08184) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BCKDK antibody (144-08184) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.