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Anti-Bcl-2 Antibody, Sodium Azide Free
Catalog #: 188-10008-SAF
Mouse anti-Human/Monkey/Pig Bcl-2 Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Does not react with mouse and rat. Other species not known. |
| Host Species | Mouse |
| Accession Number | P10415 |
| Gene Id | 596 |
| Gene Symbols | BCL2 |
| Protein Name / Synonyms | Apoptosis regulator Bcl-2 |
| Immunogen | A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | FACS: 0.5-1ug/million cells IF: 1-2ug/ml Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic, membrane |
| Research Area | Apoptosis, Autophagy |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Storage
Store the Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.