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Anti-Bcl2 Antibody, Sodium Azide Free
Catalog #: 188-11390-SAF
Mouse anti-Human Bcl2 Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | P10415 |
| Gene Id | 596 |
| Gene Symbols | BCL2 |
| Protein Name / Synonyms | Apoptosis regulator Bcl-2 |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the Bcl2 antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Western blot: 1-2ug/ml Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic, membrane |
| Research Area | Apoptosis, Autophagy |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Bcl2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. [UniProt]
Storage
Store the Bcl2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.