RayBiotech

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Anti-Beta-tubulin (166-183aa) Antibody

Catalog #: 130-10923-1000

Product Description

Specifications

Size1000 µg
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesRabbit
Accession Number
P07437
Gene Id
203068
Gene Symbols
TUBB TUBB5 OK/SW-cl.56
Protein Name / Synonyms
Beta-tubulin (166-183aa)
ClonalityPolyclonal
Immunogen
This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen Beta-tubulin (166-183aa):CTFSVVPSPKVSDTVVEPY. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography.
Immunogen Sequence
CTFSVVPSPKVSDTVVEPY
Recommended ApplicationsELISA
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Research AreaCytoskeletal Reorganization
Shipping TypeAmbient temperature
Storage-20°C

Description

Western Blot
Western blot image

130-10923 (pAb): 1:2000
10 % SDS-PAGE
Goat anti-rabbit IgG-HRP: 1:5000

Lane: M: protein marker

  1. Hela cell lysate 5 µl
  2. 293T cell lysate 5 µl
  3. Hela cell lysate 10 µl
  4. 293T cell lysate 10 µl
  5. A549 cell lysate 10 µl
  6. HepG2 cell lysate 10 µl
  7. BHK-21 cell lysate 10 µl

Western blot image

130-10923 (pAb): 1:2000
10 % SDS-PAGE
Goat anti-rabbit IgG-HRP: 1:5000

Lane: M: protein marker 5 µ

  1. Hela cell cytoplasm fraction 10 µl
  2. 293T cell cytoplasm fraction 10 µl
  3. A549 cell cytoplasm fraction 10 µl
Simple Western
Simple western image
Indirect Fluorescent Antibody (IFA) Analysis
130-10923 (1:50): Rabbit Anti-beta-tubulin
Alexa Fluor 488 goat anti-rabbit (1:200)
HepG2 cells
Subcellular Location: cytoplasm, cytoskeleton, nucleus

Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Storage/Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Expiration

12 months from the date of shipment when stored properly.