Anti-Bi-Phospho-MEK1 (S218/222) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q02750
Gene Id	5604
Gene Symbols	MAP2K1
Protein Name / Synonyms	Bi-Phospho-MEK1(S218/222)
Clonality	Polyclonal
Preparation	This MEK1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S218/222 of human MEK1.
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Western Blotting, IHC-P, Dot Blotting
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.

Specificity

Predicted Reactivity

Mouse, Rat, Chicken, Drosophila, C.Elegans, Hamster, Rabbit, Xenopus

Other Names

MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1

NCBI Accession #

NP_002746.1

Other Accession #

P36506; Q63932; P36507; Q90891; Q05116; Q01986; P29678; P31938; Q63980; Q10664; Q24324

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43439

Recommended Dilutions

DB: 1:500; IHC: 1:50-100

	Dot blot analysis of anti-Phospho-MEK1 Pab (Cat# 102-15316) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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