3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-BID Antibody, Biotinylated
Catalog #: 130-10027B-50
Rabbit anti-BID Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P55957 |
| Gene Id | 637 |
| Gene Symbols | BID |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human BID. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000(at least detecting 25 µg/ml)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
BID, apoptic death agonist is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. The expression of BID is upregulated by the tumor suppressor p53, and BID has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BID.
The major proteolytic product p15 BID allows the release of cytochrome c by similarity. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis.
The major proteolytic product p15 BID allows the release of cytochrome c by similarity. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat BID has not yet been tested.
Related Products
Goat anti BID, with HRP-conjugated secondary antibody (Cat#ER-03-0002)Recombinant Mouse BH3 Interacting Domain Death Agonist p15 (Cat#228-11484-2 )RayBio® Human Apoptosis Array 1 (2) (Cat# AAH-APO-1-2)
References
- Chou JJ, et.al.,Solution structure of BID, anintracellular amplifier of apoptotic signaling.(1999) Cell. 96(5):615-24.
- Rush J, et.al., Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. (2005) Nat Biotechnol. 23(1):94-101.
Expiration:
12 months from the date of shipment when stored properly.