Anti-Bid (BH3 Domain Specific) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P55957
Gene Id	637
Gene Symbols	BID
Protein Name / Synonyms	Bid (BH3 Domain Specific)
Clonality	Polyclonal
Preparation	This Bid antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-103 amino acids from human Bid.
Concentration (lot specific)	1.855 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.

Specificity

Other Names

BID; BH3-interacting domain death agonist; p22 BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID

NCBI Accession #

NP_001187.1;NP_001231496.1;NP_001231498.1;NP_001231499.1;NP_001231501.1;NP_932070.1;NP_932071.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

21995

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-Bid BH3 domain Pab (Cat# 102-11864) is used in Western blot to detect Bid BH3 in HL-60 cell lysate.
	Western blot analysis of anti-hBid-BH3 Pab (Cat# 102-11864) in mouse lung tissue lysates (35ug/lane). hBid-BH3(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody (Cat# 102-11864), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3) (Cat# 102-11864). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.
	Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3) (Cat# 102-11864). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.