Anti-Bim BH3 Domain Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O43521
Gene Id	10018
Gene Symbols	BCL2L11
Protein Name / Synonyms	Bim BH3 Domain
Clonality	Polyclonal
Preparation	This Bim BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-165 amino acids from human Bim BH3 Domain.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Bim belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bim contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection.

Specificity

Other Names

BCL2L11; BIM; Bcl-2-like protein 11; Bcl2-interacting mediator of cell death

NCBI Accession #

NP_001191035.1;NP_001191036.1;NP_001191037.1;NP_001191038.1;NP_001191039.1;NP_001191040.1;NP_001191041.1;NP_001191042.1;NP_006529.1;NP_619527.1;NP_619528.1;NP_619529.1;NP_619530.1;NP_619531.1;NP_61953

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22171

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-Bim BH3 domain Pab (Cat# 102-11868) is used in Western blot to detect Bim BH3 in HL-60 cell lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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