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Anti-Biotin Antibody, Sodium Azide Free
Catalog #: 188-10208-SAF
Mouse anti-Biotin Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Host Species | Mouse |
| Protein Name / Synonyms | Biotin |
| Immunogen | Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody. |
| Isotype | IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml,In situ Nucleic Acid Hybridization Western Blotting: 2-5ug/ml,Confocal Microscopy, |
| Domain / Region / Terminus | Depends on target localization |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
Storage
Store the Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.