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Anti-Biotin Antibody, Sodium Azide Free
Catalog #: 188-11609-SAF
Mouse anti-Biotin Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Host Species | Mouse |
| Protein Name / Synonyms | Biotin |
| Immunogen | Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody. |
| Isotype | IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Confocal Microscopy Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 0.5-1ug/ml,In situ Nucleic Acid Hybridization,Western: blot 1-3ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT (1) |
| Domain / Region / Terminus | target location |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Storage
Store the anti-Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.