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Anti-BLZF1 Antibody
Catalog #: 144-06430
BLZF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9H2G9 |
| Gene Id | 8548 |
| Gene Symbols | BLZF1 |
| Protein Name / Synonyms | BLZF1, GOLGIN-45, JEM-1, JEM-1s, JEM1, golgin-45 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
19kDa/44kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BLZF1 antibody (144-06430) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using BLZF1 antibody (144-06430) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using BLZF1 antibody (144-06430) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using BLZF1 antibody (144-06430) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using BLZF1 antibody (144-06430) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using BLZF1 antibody (144-06430). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.