Anti-BNIP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q12981
Gene Id	662
Gene Symbols	BNIP1
Protein Name / Synonyms	BNIP1, NIP1, SEC20, TRG-8, BCL2 interacting protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Apoptosis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

22kDa/26kDa/27kDa/31kDa

Observed MW

26kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BNIP1 (NP_001196.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using BNIP1 Antibody (144-07263) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - BNIP1 Antibody (144-07263)

Immunohistochemistry of paraffin-embedded rat brain using BNIP1 Antibody (144-07263) at dilution of 1:100 (40x lens).
Immunohistochemistry - BNIP1 Antibody (144-07263)

Expiration:

12 months from the date of shipment when stored properly.

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