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Anti-BNIP2 Antibody
Catalog #: 144-06282
BNIP2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q12982 |
| Gene Id | 663 |
| Gene Symbols | BNIP2 |
| Protein Name / Synonyms | BNIP2, BNIP-2, NIP2, BCL2 interacting protein 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
36kDa/43kDa
Observed MW
45-55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 125-280 of human BNIP2 (NP_004321.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BNIP2 antibody (144-06282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using BNIP2 antibody (144-06282). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.