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Anti-BNIP3 Antibody
Catalog #: 144-05683
BNIP3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12983 |
| Gene Id | 664 |
| Gene Symbols | BNIP3 |
| Protein Name / Synonyms | BNIP3, NIP3, BCL2 interacting protein 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
27kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human BNIP3 (NP_004043.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using BNIP3 Antibody (144-05683) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody (144-05683) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (144-05683) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using BNIP3 antibody (144-05683) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using BNIP3 antibody (144-05683) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.